Urea Page Gel. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Add 25 μl temed and 50 μl 25% aps.
TeamEPFL/Results/Toehold
For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Pour gel to ~ 0.5 cm from top. Add 25 μl temed and 50 μl 25% aps. Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays. Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. Push all the way down, but don't trap any bubbles. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Insert clean, dry comb at an angle to prevent trapping of bubbles.
Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays. Add 25 μl temed and 50 μl 25% aps. Push all the way down, but don't trap any bubbles. Insert clean, dry comb at an angle to prevent trapping of bubbles. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Pour gel to ~ 0.5 cm from top. For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels.
